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Measures

Number of red blood cells in the blood

Identifies

Red blood cell disorders

Measures: Number of red blood cells in the blood

Identifies: Red blood cell disorders

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What is the maximum days the report can be obtained for the test

The maximum days to obtain the report for the Calponin Test Immunohistochemistry is 7 days.

What are the prerequisites for the test Calponin Test Immunohistochemistry

  • Formalin-fixed, paraffin-embedded tissue sections on positively charged slides
  • Deparaffinization and rehydration of tissue sections
  • Antigen retrieval using appropriate method
  • Blocking endogenous peroxidase activity
  • Blocking non-specific protein binding
  • Incubation with primary antibody against Calponin
  • Incubation with secondary antibody conjugated to a detection system
  • Visualization of antibody binding using a chromogen
  • Counterstaining with hematoxylin, if desired
  • Mounting and coverslipping of slides

What are the measure values for the test Calponin Test Immunohistochemistry

  • Positive: Strong cytoplasmic staining in smooth muscle cells
  • Negative: Absence of staining in smooth muscle cells
  • Intensity: 0 (no staining), 1+ (weak), 2+ (moderate), 3+ (strong)
  • Distribution: Focal, multifocal, diffuse

What does this test Calponin Test Immunohistochemistry identify?

  1. Calponin Test Immunohistochemistry identifies the presence of calponin protein in tissue samples.
  2. Calponin is a cytoskeletal protein that is involved in smooth muscle contraction and cell motility.
  3. This test is commonly used in the diagnosis of smooth muscle tumors, such as leiomyosarcoma, leiomyoma, and gastrointestinal stromal tumors.
  4. Positive staining for calponin in immunohistochemistry indicates the presence of smooth muscle cells in the tissue sample.

Why is this test Calponin Test Immunohistochemistry taken?

  1. Calponin Test Immunohistochemistry is taken to diagnose smooth muscle tumors, as calponin is a specific marker for smooth muscle cells.
  2. It is used to differentiate between smooth muscle tumors and other types of tumors, such as gastrointestinal stromal tumors (GIST) or leiomyosarcomas.
  3. Calponin Test Immunohistochemistry is also helpful in determining the prognosis of patients with smooth muscle tumors, as the expression of calponin can be correlated with tumor aggressiveness.
  4. Additionally, this test can be used to monitor the effectiveness of treatment in patients with smooth muscle tumors, as changes in calponin expression levels may indicate response to therapy.

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